While the ability to reproduce published results is generally recognized as a defining characteristic of science, in recent years there has been a growing realization many published biomedical research findings cannot be repeated outside of the laboratories that originally produced them.
In the news:
Selected papers addressing reproducibility issues
Begley, C.G. and L.M. Ellis,
Drug development: Raise standards for preclinical cancer research. Nature, 2012. 483(7391): 531-3. Prinz, F., T. Schlange, and K. Asadullah,
Believe it or not: how much can we rely on published data on potential drug targets? Nat Rev Drug Discov, 2011. 10(9): 712. Carp, J.,
Better living through transparency: improving the reproducibility of fMRI results through comprehensive methods reporting. Cogn Affect Behav Neurosci, 2013. 13(3): 660-6. Collins, F.S. and L.A. Tabak,
Policy: NIH plans to enhance reproducibility. Nature, 2014. 505(7485): 612-3. [PMCID:PMC4058759]. McNutt, M.,
Raising the bar. Science, 2014. 345(6192): 9. Chruszcz, M., et al.,
Unmet challenges of structural genomics. Curr Opin Struct Biol, 2010. 20(5): 587-97. [PMCID:PMC3017768]. Cheng, Q.D., et al.
Effect of the weather conditions during solution preparation on lysozyme crystallization J. Appl. Cryst. (2017). 50, 1341-1351 link Solutions
The Center for Open Science. [cited 2014 October 9, 2014]; Available from:
http://centerforopenscience.org. Majorek KA, Kuhn ML, Chruszcz M, Anderson WF, Minor W
Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments.
Protein Sci. 2014. 23(10):1359-68. Times cited: 12. [Pub Med ID: 25044180] [Pub Med Central ID: PMC4286991] Zimmerman MD, Grabowski M, Domagalski MJ, Maclean EM, Chruszcz M, Minor W
Data management in the modern structural biology and biomedical research environment.
Methods Mol. Biol. 2014. 1140:1-25. Times cited: 6. [Pub Med ID: 24590705] [Pub Med Central ID: PMC4086192] Zheng H, Chruszcz M, Lasota P, Lebioda L, Minor W
Data mining of metal ion environments present in protein structures.
J. Inorg. Biochem. 2008. 102(9):1765-76. Times cited: 124. [Pub Med ID: 18614239] [Pub Med Central ID: PMC2872550 Tools to determine the quality of the structure and/or check if there is a potential purification artifact
Covid-19.bioreproducibility.org Validated SARS-CoV-2 related structural models of potential drug targets HKL3000 - This program package allows for lattice parameter search against PDB
molstack - This tool allows the visualization and analysis of multiple sets of coordinate and electron density data [PMID: 28815771]
ContaMiner - This is a webserver for early identification of unwantedly crystallized protein contaminants by molecular replacement using structures of known artifacts [PMC5140001]
SAUC - Search of Alternate Unit Cells - This server allows to search PDB for cells representing similar lattices