Protein Science (2016) 25(3): 720-33. DOI: 10.1002/pro.2861

Protein purification and crystallization artifacts: the tale usually not told
Ewa Niedzialkowska, Olga Gasiorowska, Katarzyna B. Handing, Karolina A. Majorek, Przemyslaw J. Porebski, Ivan G. Shabalin, Ewelina Zasadzinska, Marcin Cymborowski, Wladek Minor


List of PDB codes that can be used to detect crystallization artifacts using MR

If you would like to add a new purification or crystallization artifact to this list, please contact us and include a sequence or UniProt ID.
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  Name of the protein Molecular weight [kDa] PDB ID Reference
Affinity, solubility, anti-aggregation tags Maltose-binding protein (MBP) 43 1LLS, 1MPB, 3PUW, 3SEU, 4KYC,  
  Glutathione-S-transferase (GST) 24 4ECB  
  Thioredoxin (Trx) 11 1F6M, 2AJQ, 2H73, 4HU9, 4X43,  
  N-Utilization substance (NusA) 55 1U9L[1], 1WCN[2], 2KWP[3], 4MTN[4]  
  Small ubiquitin related modifier 1 (SUMO1) 12 2UYZ, 1Z5S, 4WJQ, 2IO2  
  Haloalkane dehalogenase 33 4E46  
E. coli native proteins Metal-binding lipocalin (YodA) 25 1OEJ, 4TNN, PMID: 16814929
  Carbonic anhydrase (YadF) 25 2ESF PMID: 16814929
  Ferric uptake regulator (Fur) 16 2FU4 PMID: 16814929
  cAMP-regulatory protein (CRP) 24 1CGP, 2CGP, 2GZW, 3FWE, 3HIF, 3N4M, 3QOP, 4FT8, 4HZF, 4I0A, 4I0B, 4N9H, 4N9I, PMID: 16814929
  Glucosamine-6-phosphate synthase (GlmS) 67 4AMV, 1JXA, 3OOJ, 2J6H PMID: 16814929
  Glycogen synthase (GlgA) 53 2QZS PMID: 16814929
  Component 1 of the 2-oxoglutarate dehydrogenase complex (ODO1) 105 2JGD PMID: 16814929
  Component E2 of the dihydrolipoamide succinyltransferase (ODO2) 44 1C4T PMID: 16814929
  Formyl transferase (YfbG) 46 1U9J, 1YRW, 1Z7E, 2BLN 4WKG PMID: 16814929
  Cu/Zn-superoxide dismutase (Cu/Zn-SODM) 16 1ESO  
  Chloramphenicol-O-acetyl transferase (CAT) 26 1Q23  
  Host factor-I protein (Hfq) 11 3VU3 PMID: 27577872
  Gab protein 37 1JR7 PMID: 18391407PMID: 27577872
  Glycerol dehydrogenase (GlyDH) 39 5XN8 PMID: 28695861
Proteases Tobacco etch virus (TEV) 28 1LVB, 1LVM  
  Rhinovirus 3C protease 48 1CQQ, 2XYA  
  SUMO protease C-terminal domain 26 2HL9  
  Enterokinase 26 1EKB  
  Trypsin 26 3UY9  
  Chymotrypsin 26 1GGD  
  Thrombin (active form) 36 3SQE, 1MH0, 4H6T  
  Thermolysin 60 4D9W  
  Proteinase K 40 3DVS  
  Pepsin 41 5PEP  
  Neutrophil elastase 29 5ABW  
  LysN Peptidyl-Lys metalloendopeptidase 44 1GE7  
  LysC Lysyl endopeptidase 28 4NSY  
  Factor Xa 55 1KIG  
Exogenous proteins Lysozyme 16 4TWS, 4PRQ, 1AKI

PMC: 3274391

  DNase protein 31 2A40  

Footnotes

[1] crystal structure of the C-terminal fragment of NusA from E. coli,
[2] NMR structure of the C-terminal fragment of NusA from E. coli,
[3] NMR structure of the N-terminal fragment of NusA from E. coli,
[4] homologous structure from P. limnophilus,